Born: October 24, 1503, Lisbon, Portugal
Died: May 1, 1539 (age 35 years), Toledo, Spain
Spouse: Charles V (m. 1526–1539)
Parents: Manuel I of Portugal and Maria of Aragon, Queen of Portugal
Siblings: John III of Portugal, Miguel da Paz, Prince of Portugal, Henry, King of Portugal, and more
Grandparents: Isabella I of Castile, Ferdinand II of Aragon, Beatriz of Portugal, Duchess of Viseu, and more